Regulatory
Part:BBa_K3239005:Design
Designed by: Xingyu Zhou Group: iGEM19_ShanghaiFLS_China (2019-10-15)
Prm1 transcription factor in the P.pastoris GS115 strain
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1034
Illegal PstI site found at 1556 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1034
Illegal NheI site found at 2815
Illegal PstI site found at 1556 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1034
Illegal BglII site found at 387
Illegal BamHI site found at 1917
Illegal BamHI site found at 2836 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1034
Illegal PstI site found at 1556 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1034
Illegal PstI site found at 1556 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Nucleotide 2811 (A) is changed to (G) for Type IIS compatiblility.
Source
P. pastoris GS115